Sensitivity and specificity of Indirect Enzyme Linked Immuno sorbent Assay (ELISA) for diagnosis of hydatidosis in dromedary camels using hydatid cyst fluid antigens.
نویسندگان
چکیده
منابع مشابه
Application of a modified human enzyme-linked immunosorbent assay kit for diagnosis of hydatidosis in sheep
Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for epidemiologic studies. The present study was performed with a commercial human en...
متن کاملHigh sensitivity molecular detection with enzyme-linked immuno-sorbent assay (ELISA)-type immunosensing.
Here we describe an immunosensing method, which is designed for high sensitivity sensing of various substances utilizing specificity of antigen-antibody (ELISA-type) interaction. The building up of the nanostructured sensing interface and the immunointeraction at the surface were characterized by atomic force microscopy. The proposed design makes potentially feasible attaining ultimate single-m...
متن کاملEvaluation of Enzyme Linked Immunosorbent Assay, Utilizing Native Antigen B for Serodiagnosis of Human Hydatidosis
Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...
متن کاملEVALUATION OF THE ENZYME-LINKED IMMUNO-ELECTRO TRANSFER BLOT (EITB) TECHNIQUE USING HYDATID CYST ANTIGENS B/5 AND TOTAL IgG ANTIBODIES IN LABORATORY DIAGNOSIS OF HUMAN HYDATIDOSIS
Objective: To evaluate the validity of the Enzyme-linked Immuno-electro Transfer Blot (EITB) technique to diagnose human hydatidosis using sheep hydatid fluid antigens and human sera infected with hydatidosis. Design: After preparing parasite antigen from sheep hydatid cyst fluid, all collected human sera infected with hydatidosis and other parasitic diseases as well as normal individuals, were...
متن کاملDevelopment of an enzyme-linked immuno-sorbent assay (ELISA) method for carbofuran residues.
The haptens 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]butanoic acid (BFNB) and 6-[((2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)-carbonylamino]hexanoic acid (BFNH) were synthesized and then used to develop a rapid,specific and sensitive ELISA method to determine residues of the pesticide carbofuran in a variety of matrices. A hybridoma cell line (5D3) producing anti-carbofu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Medical Research
سال: 2020
ISSN: 2357-0520
DOI: 10.21608/jvmr.2020.69696